Effects of a Natural Environment on Caenorhabditis elegans

The nematode Caenorhabditis elegans is an important model organism in the field of biology and yet little is known about how it lives in its natural environment. In order to study C. elegans in a more naturalistic setting, we created artificial soil microcosms. Populations of C. elegans were estimated over time by taking small periodic samples. We were able to maintain large, stable populations for over one month, or twelve generations. This methodology could be a useful tool in many areas of C. elegans biology. We are investigating the effects that this naturalistic environment has on a known aging mutant, daf-2. The effect of this mutation, although well characterized in a laboratory setting to have an extension in lifespan, is unknown in a natural environment. Artificial soil microcosms will aid future C. elegans studies in determining the effects of a natural setting on lab-characterized traits.